General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen Motilin Receptor Membrane Preparation, 1 vial. (HTS121M)
The ChemiScreen Motilin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MLNR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay Motilin Receptor Frozen Cells, 2 Vials of Cells. (HTS121RTA)
The Ready-to-Assay Motilin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MLNR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay BB1 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS123RTA)
The Ready-to-Assay BB1 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line, 2 vials. (HTS117C)
The ChemiScreen M4 Muscarinic Acetylcholine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen GABAB Receptor Membrane Preparation, 1 vial. (HTS119M)
The ChemiScreen GABAB Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of GABBR1 - GABBR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen FFA2/GPR43 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS063C)
The ChemiScreen FFA2/GPR43 Free Fatty Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx cAMP Hunter CHO-K1 AVPR2 Gs Cell Line, 2 vials. (95-0150C2)
The cAMP Hunter AVPR2 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type AVPR2, a naturally Gs coupled GPCR. Upon activation of AVPR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS IL27RA/IL6ST Dimerization Cell Line, 2 vials. (93-1119C3)
The PathHunter IL27RA/IL6ST Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-27) induced dimerization of IL27RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL27RA target protein fused to EA. Upon ligand-induced dimerization of IL27RA/IL6ST, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Eurofins Discoverx cAMP Hunter CHO-K1 EBI2 Gi Cell Line, 2 vials. (95-0172C2)
The cAMP Hunter EBI2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. hydroxy/dihydroxycholesterol) based activation of EBI2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type EBI2, a naturally Gi/Go coupled GPCR. Upon activation of EBI2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
VITRO BIOPHARMA HUM LUNG SQUAMOUS CELL CARCINO
Human Lung Squamous Cell Carcinoma Cancer Associate Fibroblasts: 1,000,000 Cells Per Vial
